Cryptosporidium (Fecal), Human, BioAssay(TM) ELISA Kit

Cryptosporidium (Fecal), Human, BioAssay(TM) ELISA Kit is an in vitro immunoassay for the qualitative determination of Cryptosporidium antigen in feces. It is a double antibody (sandwich) ELISA using an anti-Cryptosporidium antibody to capture the antigen from the stool supernatant. A second anti-Cryptosporidium antibody is then added which sandwiches the captured antigen. This reaction is visualized by the addition of an anti-second antibody conjugated to peroxidase and the chromogen tetramethylbenzidine (TMB). The resulting blue color development indicates the presence of Cryptosporidium antigens being bound by the anti-Cryptosporidium antibodies.\n\nBackground:\nCryptosporidium is a coccidian parasite that is recognized as an important enteric pathogen. The organism causes an acute, though self-limiting infection in immunocompetent individuals. Incubation periods of 1 to 12 days have been reported with most oocyst shedding ending by day 21. Symptoms range from mild to severe diarrhea with a variety of complications. The infection in immunocompromised patients is much more severe and may often be life threatening. Passage of fluid, up to 12 liters per day, has been reported. Multiple pathways of Cryptosporidium transmission have been implicated. These include animal to human, water contamination and person-to-person. The latter may include contact between members of the same household, day care centers, and homosexual men. Identification of Cryptosporidium infections was done originally by direct detection techniques. Of these, microscopic examination of stools using stains or fluorescence labeled antibodies has been the most common. However, this method relies on an experienced technician and subsequent observation of intact organisms. Because of the historically low proficiency of correct microscopic examinations, alternative diagnostic methods have been investigated. One important alternative has been the development of an antigen capture ELISA for use with stools. These tests, which have shown comparable sensitivity to experienced microscopic examinations, are fairly simple to perform and do not require the observation of intact organisms.\n \nKit Components: \n1. Microtiter Plate: 1X96wells \n2. Reagent 1: 1x11ml \n3. Reagent 2 1x11ml \n4. Positive Control: 1x2ml \n5. Negative Control: 1x2ml\n6. Tetramethylbenzidine (TMB): 1x11ml\n7. Dilution Buffer: 2x30ml\n8. Wash Buffer, 20X: 1x25ml \n9. Stop Solution: 1x11ml\n \nStorage and Stability:\nStore all components at 4 degrees C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7944-17

Size

1Kit

Applications

ELISA

Reactivities

Hum

References

1. Chapman, P.A. \"Cryptosporidiosis: Recent Trends in Epidemiology, Diagnosis, and Treatment.\" Serodiag & Immunother Infect Dis #2, 1988, pp. 311-317. 2. Meyer, E.A. \"Waterborne Giardia and Cryptosporidium.\" Parasit Today. 4: 7, 1988, pp. 200-201. 3. Garcia, L., et al., ACPR, May 1988, pp. 38-41. 4. Stibbs, H., et al., J Clin Micro, 24: 4, Oct. 1986, pp.517-521. 5. McLaughlin, J. et al. \"Indentification of Cryptosporidium Oocysts by Monoclonal Antibody.\" Lancet, January 3, 1987, pp.51. 6. Ungar, B. \"Enzyme-Linked Immunoassay for Detection of Cryptosporidium Antigens in Fecal Specimens.\" J Clin Micro, 28: 11, Nov 1990, pp. 2491-2495. 7. Anusz, K., et al. \"Detection of Cryptosporidium parvum Oocysts in Bovine Feces by Monoclonal Antibody Capture Enzyme-Linked Immunosorbent Assay.\" J. Clin Micro, 28: 12, dec. 1990, pp. 2770-2774. 8. Jokipii, L., et al. \"Cryptosporidium: A Frequent Finding In Patients With Gastrointestinal Symptoms.\" Lancet, August 13, 1983, pp. 358-360. 9. Shephard, R., et al. \"Shedding of Oocysts of Cryptosporidium in Immunocompetent Patients.\" J Clin Pathol, 41 1988, pp. 1104-1106. 10. Holten-Anderson, W., et al. \"Prevelence of Cryptosporidium Among Patients with Acute Enteric Infection.\" J. Infect, 9 1984, pp. 277-282. 11. Jokipii, L. and Jokipii, M. \"Timing of Symptoms and Oocyst Excretion in Human Cryptosporidiosis.\" N Engl J Med, 315: 26, 1986, pp.1643-1647. 12. Egger, M., et al. \"Symptoms and Transmission of Intestinal Cryptosporidiosis.\" Arch Dis Child, 65 pp 445-447.\n13. Hart, M., et al. \"Acute Self-Limited Colitis Associated with Cryptosporidium in an Immunocompetent Patient.\" J Ped Gastro Nutr, 8 1989, pp. 401-403. 14. Nwanyanwu, O., et al. \"Cryptosporidiosis in a Day-Care Center.\" Texas Med, 85 June 1989, pp. 40-43. 15. Sloan, L.M., and Rosenblatt, J.E. \"Evaluation of Enzyme-Linked Immunosorbent Assay for Detection of Cryptosporodium spp. in Stool Specimens.\" J Clin Micro, 31: 6, June 1993, pp. 1468-1471. 16. Current, W. and Garica, L. \"Cryptosporidiosis.\" Clin Micro Rev, 4: 3, July 1991, pp. 325-358. 17. Weber, R. et al. \"Threshold of Detection of Cryptosporidium Oocysts in Human Stool Specimens; Evidence for Low Sensitivity of Current Diagnostic Methods.\" J Clin Micro, 29: 7, July 1991, pp. 1323-1327.