Crystallin AB (Alpha crystallin B chain, CryAB, Alpha (B)-crystallin, Rosenthal fiber component, Heat-shock protein beta-5, HspB5, CryA2, AACRYA, Alpha crystallin B chain, CRYA2, Crystallin alpha polypeptide 2, CTPP 2, CTPP2, Heat shock 20kD like protein,

AlphaB Crystallin (CRYAB, also known as Crystallin alpha-B chain and HspB5) is a 22-23kD member of the HSP20 family of proteins. It has widespread expression in many tissues in addition to lens epithelium, where it noncovalently oligomerizes with CRY-alphaA to generate a transparent 350-1000kD alpha-Crystallin protein complex. Human CRYAB is 175aa in length. There is a alpha-Crystallin Hsp domain over aa66-149. Multiple posttranslational modifications may exist. The N-terminal Met and MetAspIleAlaIleHis sequence is occasionally cleaved. There is also phosphorylation at Ser45 and 59, potential O-GlcNAc modification at Thr158, 162 or 170, and acetylation at Lys92. There is an alternate start site at Met68 that may be accompanied by a 47aa substitution for aa109-175. Full-length human CRYAB is 54% aa identical to CRY-AlphaA, and 98% aa identical to mouse CRYAB. \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 0.1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-28C: IgG, H&L (HRP) Pab DkxGt\nI1904-28G: IgG, H&L (HRP) (X-Adsorbed) Pab DkxGt\nI1904-28K: IgG, H&L (HRP) Pab RbxGt\nI1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab RbxGt\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS, 0.02% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7944-60C

Size

100ug

Applications

WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 100ul sterile PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

Additional Info

Recognizes human Crystallin AB (CRYAB) at ~23kD. Does not crossreact with recombinant human CRYAA in Western Blots. Species Crossreactivity: mouse and rat