Alpha B-crystallin is a heat shock chaperone protein, up-regulated by stress. It has been shown to be expressed in the vertebrate eye lens and also found in cardiac and skeletal muscles, the kidney and the brain. Alpha B-crystallin is also found to be a component of Rosenthal fibers and a consistent tissue marker of epileptic foci.
Applications:
Suitable for use in Western Blot. Other applications have not been tested.
Recommended Dilutions:
Western Blot: 0.5ug/ml detected alpha B-crystallin on 10ug of human heart tissue lysate.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.