Crystallin, alpha B, Recombinant, Human

Alpha crystallins are composed of two gene products; alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein family (sHSP also known as HSP20). They act as molecular chaperones and hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha-crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-B is expresssed widely in many tissues and organs and occurs in many neurological diseases.\n\nRecombinant protein corresponding to human Crystallin, alpha B expressed in E. coli (NP_001876).\n\nAA Sequence:\nMDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFPT STSLSPFYLR PPSFLRAPSW\nFDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHR KYRIPADVDP LTITSSLSSD GVLTVNGPRK QVSGPERTIP ITREEKPAVT AAPKK\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7944-53

Size

500ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 50mM sodium chloride, 1mM EDTA.

Purity

~95% by SDS PAGE; Purified by using conventional chromatography techniques.

References

1. Bera, S., et al., Biochemistry 41(52): 15,876-15,882 (2002). 2. Reddy, G.B., et al., FEBS Lett. 522(1-3): 59-64 (2002). 3. Kamradt, M.C., et al., J. Biol. Chem. 277(41): 38,731-38,736 (2002). 4. Ito, H., et al., J. Biochem. 131(4): 593-603 (2002). 5. Hasan, A., et al., Biochemistry 41(52): 15,876-15,882 (2002).