CSK Binding Protein (CBP, Csk Binding Protein, PAG, Protein Associated with Glycosphingolipid Enriched Microdomains)

Csk binding protein, also known as PAG (phosphoprotein associated with GEMs), is a ubiquitously expressed 46kD transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80kD molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.\n\nPositive Control: RAMOS and Jurkat cells\n\nApplications: \nSuitable for use in Flow Cytometry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2098-09A

Size

100ug

Applications

FC, WB

Hosts

Mouse

Reactivities

Hum

Form

15mM Sodium Azide in PBS. pH 7.4

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG2a

References

1. Brdickova N et al. Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton. FEBS Lett 507:133-6 (2001).\n2. Brdicka T et al. Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation. J Exp Med 191:1591-604 (2000).

Additional Info

This antibody recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain. Species Crossreactivity: Crossreacts with Human. Does not react with mouse, rat, or bovine.

Alternative Names

CBP, Csk Binding Protein, PAG, Protein Associated with Glycosphingolipid Enriched Microdomains