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Csk (C-Terminal Src kinase)

Cat no: C7945

Csk (C-Terminal Src kinase)

Csk (C-terminal c-src kinase) is an ubiquitously expressed tyrosine kinase that negatively regulates members of the Src-family of protein kinases through phosphorylation of a carboxy-terminal tyrosine residue. Csk is cytosolic but translocates to the membrane after association with a 36kD protein. Csk then interacts with its physiologically relevant substrates.\n\nApplications: \nSuitable for use in Immunoblot and Immunocytochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nImmunoblot Analysis: 1ug/ml detects Csk in RIPA lysates from Hut-78, TF-1, K-562 cells. TF-1cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with 1ug/ml anti-Rat p50 Csk. Proteins were visualized using a goat a-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. \n\nImmunocytochemistry: 20ug/ml gave positive immunostaining for Csk in TF-1 cells fixed with 1% paraformaldehyde.\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7945

Size

200ug

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 40%.

P Type

Pab

Purity

Purified by Protein A chromatography.

Isotype

IgG

References

Application: 1. Ford, C.E., J. Biol. Chem. 269: 30,378

Additional Info

Recognizes Csk (50kD). Species crossreactivity: Human, mouse and bovine.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info
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