CSN5 (JAB1, SGN5, MOV-34, MGC3149, COPS5, COP9 signalosome complex subunit 5, JAB-1, Jun Activating Domain-Binding Protein, KIC2, Kip1 C-terminus interacting protein 2, Sgn5)

JAB-1 is a 38kDa protein which was originally described as a coactivator for cJUN. It is present in a large protein complex called the Signalosome, which has been proposed to play a signaling role in mammalian cells. This protein complex appears to be analogous to the COP9 complex in plants, which is important in light-mediated plant development. Although the specific function of JAB-1 in this complex is unclear, it has recently been implicated in targeting proteins for degradation by the proteasome.\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5ug/ml\nImmunoprecipitation: 0.5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nRecombinant GST-JAB-1 protein, 293 cells.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7945-54B

Size

100ul

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.4. Preservative free.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a,k

References

1.Boussiotis, V.A., et al. (2000) p27kip1 functions as an anergy factor inhibiting interleukin 2 transcription and clonal expansion of alloreactive human and mouse helper T lymphocytes. Nat Med, 6, 290-297. 2.Bianchi, E., et al. (2000) Integrin LFA-1 interacts with the transcriptional co-activator JAB1 to modulate AP-1 activity. Nature, 404, 617-621. 3.Tomoda, K., et al. (1999) Degradation of the cyclin-dependent-kinase inhibitor p27Kip1 is instigated by Jab1. Nature 398: 160-165.\n4 .Wei, N., et al. (1998) The COP9 complex is conserved between plants and mammals and is related to the 26S proteasome regulatory complex. Current Biology 8: 919-922. 5.Seeger, M., et al. (1998) A novel protein complex involved in signal transduction possessing similarities to 26S proteasome subunits. FASEB J 12:469-478. 6.Claret, F.X., et al. (1996) A new group of conserved coactivators that increase the specificity of AP-1 transcription factors. Nature 383:453-457. 7.Wei, N., et al. (1994) Arabidopsis COP9 is a component of a novel signaling complex mediating light control of development. Cell 78:117-124.

Additional Info

Recognizes mouse JAB-1. Species Crossreactivity: human

Alternative Names

JAB1, SGN5, MOV-34, MGC3149, COPS5, COP9 signalosome complex subunit 5, JAB-1, Jun Activating Domain-Binding Protein, KIC2, Kip1 C-terminus interacting protein 2, Sgn5