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CtBP1

Cat no: C7947

CtBP1

Applications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1ug/ml detects CtBP-1 in nuclear extract from HeLa cells, as well as in RIPA lysates from 3T3/A31, A431 and Jurkat cells. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with C7947 (1ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.\nImmunoprecipitation: Reported to immunoprecipitate CtBP-1 from transfected cells. \nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb\nI1904-46Q: IgG, H&L (HRP) Pab Gt xRb\n\nPositive Antigen Control: \nH1838: HeLa Nuclear Lysate. Add an equal volume of Laemmli reducing sample buffer to 5-10ul of lysate and boil for 5 minutes to reduce the preparation. Load 10-20ug of reduced lysate per lane for minigels.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7947

Size

200ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide, before the addition of glycerol to 30%

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Zhang, Q., et al., Science 295: 1,895-1,897, 2002. 2. Schaeper, U., et al., Proc. Natl. Acad. Sci. USA 92: 10,467-10,471, 1995.

Additional Info

Recognizes human CtBP-1, Mr 53kD. Species Crossreactivity: Weak in mouse.

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Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info
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