CTBS (Di-N-acetylchitobiase; also chitobiase) is a 40kD lysosomal glycoprotein that belongs to glycosyl hydrolase family # 18. It is a reducing-end glycoside hydrolase that serves to degrade asparagines-linked carbohydrates. Mature human CTBS is 347 amino acids (aa) in length and contains one catalytic domain (aa 115-357). There are multiple potential isoforms of CTBS. One shows a three aa deletion of aa 30-32, accompanied by a two two aa substitution for the C-terminal 279 amino acids. There are two potential alternate start sites, one at Met92 and a second at Met207. Mature human CTBS (aa 39-385) is 85% aa identical to mouse CTBS.
Applications:
Suitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Direct ELISA: 0.5-1.0ug/ml detection limit for rhCTBS is ~ 0.2ng/well.
Western Blot: 0.1-0.2ug/ml detection limit for rhCTBS is ~ 10ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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