CTGF is a heparin-binding pleiotropic growth factor involved in chondrogenesis and potentially skeletogenesis. CTGF promotes the adhesion of epithelial cells and fibroblasts, stimulates integrin expression, and also promotes endothelial cell survival, migration and adhesion, implicating CTGF in angiogenesis. CTGF induces extracellular matrix formation, including the deposition of excessive collagen in fibrotic diseases, and has also been implicated in wound healing.
Applications:
Suitable for use in ELISA, Functional Assays, and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 0.5-2ug/ml
Western Blot: 0.1-0.2ug/ml
Functional Assays: 6-9ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C for short-term only.Stable for 12 months at -20 degrees C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.