CTHRC1 (Collagen triple helix repeat-containing protein 1) is a 28-30kD, secreted glycoprotein that bears similarity to the C1q/TNF-alpha-related family of proteins. It is expressed by disparate cell types, including renal epithelium, neurons, osteoblasts and smooth muscle cells. Functionally, it is recognized to be induced by BMP-2, and to block TGF-b-induced collagen type I and III synthesis. Mouse CTHRC1 is 245aa in length. It contains a 32aa signal sequence, a 16aa prosegment (aa33-48), and a 197aa mature region that shows one collagen-like domain (aa59-92). Proteolytic processing may generate multiple CTHRC1 isoforms. There is the potential for an intracellular full-length 33kD, 245aa form, plus extracellular isoforms that are 28kD (aa33-245), 26kD (aa49-245), 20kD (aa98-245) and 18kD and 16kD in size, the last two representing variants of the 20kD form with C-terminal processing. CTHRC1 may undergo dimerization, trimerization and oligomerization. One mouse splice variant shows a deletion of aa53-126. Full-length mouse CTHRC1 shares 99% and 93% aa identity with rat and human CTHRC1, respectively.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions:
Western Blot: 0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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