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CTP Phosphocholine cytidylyltransferase (CCT alpha, CCTa)

CTP Phosphocholine cytidylyltransferase (CCT alpha, CCTa)

Cat no: C7948-35

Supplier: United States Biological
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CTP:phosphocholine cytidylyltransferase (CCT) is a critical enzyme that regulates the CDP-choline pathway for the biosynthesis of phosphatidylcholine. Three distinct CCT isoforms are found in mammals, including CCTa, CCTB2, and CCTB3 (1,2). CCTa is the major isoform that is expressed in most tissues (3). CCTa is essential in the synthesis and secretion of surfactant by alveolar epithelial cells and is important in maintaining the phosphatidylcholine level that regulates lipoprotein as- sembly and secretion in hepatocytes (4,5). CCTa is a major component in membrane biogenesis during cytokine secretion by stimulated macrophages (6). Monoubiquitination of CCTa prevents it from entering the nucleus and leads to its degradation by lysosome (7). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C7948-35
Reactivities: Human
Hosts: Rabbit
Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied in 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A affinity chromatography.
References: 1. Jackowski, S. and Fagone, P. (2005) J Biol Chem 280, 853-6, 2. Clement, J.M. and Kent, C. (1999) Biochem Biophys Res Commun 257, 643-50, 3. Karim, M. et al. (2003) Biochim Biophys Acta 1633, 1-12, 4. Tian, Y. et al. (2007) Mol Cell Biol 27, 975-82, 5. Jacobs, R.L. et al. (2004) J Biol Chem 279, 47402-10, 6. Tian, Y. et al. (2008) J Cell Biol 181, 945-57, 7. Chen, B.B. and Mallampalli, R.K. (2009) Mol Cell Biol 29, 3062-75.
Additional info: Recognizes human CCT Alpha.

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