CTP:phosphocholine cytidylyltransferase (CCT) is the key regulator enzyme in the synthesis of phosphatidylcholine (PC), the most abundant phospholipid in eukaryotic cell membranes. CCT catalyzes the transfer of a cytidylyl group from CTP to phosphocholine to form CDP-choline. A steady, albeit low, production of PC is necessary to maintain suitable PC content for lipid homeostasis. However, elevated activity of CCT is required during cell division when increased PC production is needed for membrane biogenesis. To date, three CCT isoforms have been identified: CCT-alpha, CCT-beta 1, and CCT-beta 2. CCT-alpha is localized to the nucleus and nuclear envelope in many cell types. An exception is lung-derived cell, where CCT-alpha is localized primarily in cytoplasmic and ER compartments. CCT-alpha is involved in important nuclear functions such as coordinating PC synthesis with cell-cycle regulation and regulating proliferation of the nucleoplasmic reticulum, an intranuclear membrane network implicated in calcium signaling and nuclear-cytoplasmic transport.
Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions:
Western Blot: 1:1000-1:10,000
Immunohistochemistry: 1:50-1:100
Immunocytochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
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