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Cullin 1 (CT) (CUL-1)

Cullin 1 (CT) (CUL-1)

Cat no: C7949-01H

Supplier: United States Biological
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Cullin proteins assemble a large number of RING E3 ubiquitin ligases and regulate various physiological processes. It has been shown that the SKP1, CUL1, F-box (SCF) containing pathway, although similarly used by the mammalian CUL1, is not shared by other cullin members. This implies that most cullins may use a SKP1/F-box-independent pathway to facilitate protein degradation. The majority of CUL1 has been found in a complex with CAND1 and ROC1, independent of SKP1 and F-box protein SKP2. Data suggests that CAND1 regulates the formation of the SCF complex, and its dissociation from CUL1 is coupled with the incorporation of F-box proteins into the SCF complex, causing their destabilization. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:2,000-1:10,000 Western Blot: 1:500-1:1,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C7949-01H
Reactivities: Human
Hosts: Rabbit
Applications: ELISA, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid, 0.01% sodium azide.
P type: Pab
Purity: Purified by delipidation and defibrination.
References: 1. Michel, J.J., et al., (1998), "Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A", Cell Growth Differ., 9 (6), 435-449. 2. Kipreos, E. T., et al., (1996), "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family", Cell, 85 (6), 829-839. 3. Ohta, T., et al., (1999), "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity", Mol. Cell., 3 (4), 535-541. 4. Mathias, N., et al., (1996), "Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins", Mol. Cell Biol., 16:12, 6634-6643.
Additional info: Recognizes human Cullin 1.

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