Cullin-1 is a member of the family of human cullin genes (cullin-1, 2, 3, 4a, 4b, and 5) homologous to the s. cerevisiae cdc53 gene. Cullin-1 forms a complex with human p19skp1 and F box protein p45skp2. Both of which, together with cdc53, as three subunits, form an ubiquitin ligase controlling proteolysis of G1 cell cycle regulatory proteins. The cullin-1/p19skp1/p45skp2 complex is thought to play a role in the ubiquitination of G1 regulatory proteins.
Applications:
Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1-2ug/ml for 2h at RT
Immunoprecipitation: 10ug/mg protein lysate
Immunohistochemistry: Formalin fixed paraffin embedded
Optimal dilutions to be determined by the researcher.
Positive Control:
Raji cells, tonsil
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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