Cullins (1, 2, 3, 4a, 5 and 7) are a family of proteins that assemble a large number of ubiquitin ligases to catalyse polyubiquitiation, as well as binding to various specific factors to recruit substrates. Cullin 2 (CUL 2) is a component of E3 ubiquitin ligase complexes which controls the proteolysis of G1 cell cycle regulatory proteins. It forms a complex with an inactive transcriptional elongation complex formed by three subunits elongain C, elongain B and VHL.
Applications:
Suitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
ELISA: 1:2000-1:10,000
Western Blot: 1:500-1:1000
Immunohistochemistry (Formalin fixed paraffin embedded): 1:500
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Quick Search
Products 
