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Cullin 3 (CUL-3)

Cullin 3 (CUL-3)

Cat no: C7949-20B

Supplier: United States Biological
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Involved in ubiquitination of cyclin E and, in complex with RBX1, of cyclin D1 (in vitro) thus involved in regulation of G1/S transition. Seems not be part of a SCF complex with SKP1. In complex with SPOP, involved in ubiquitination of both BMI1/PCGF4 and H2AFY. Applications: Suitable for use in Flow Cytometry, Western Blot, Dot Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-2ug/ml Western Blot: 0.2-2ug/ml Immunocytochemistry: 2-100ug/ml Immunoprecipitation: 100-500ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C7949-20B
Reactivities: Human, Mouse, Rat
Hosts: Mouse
Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
Additional info: Recognizes human Cullin 3. Species Crossreactivity: mouse, rat

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