Cullin 3 is a member of the family of human Cullin genes (CUL-1, -2, -3, -4a, -4b and -5) homologous to the S. cerevisiae cdc53 gene.
Cullin 3 forms a tight complex with cyclin E and targets it for ubiquitination, which leads to its degradation by proteosomes.
Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.