Cullin 3 (CUL-3)

Two distinct members of the Cullin gene family- Cul3 and Cul11- have been implicated in the ubiquitin-mediated destruction of mammalian cyclin E. Cul3 may be a core component of the E3 ubiquitin ligase complex that targets free cyclin E for destruction. Cul3 physically associates with cyclin E in mammalian cells, as would be expected if Cul3 was a component of the cyclin E ubiquitin ligase. Overexpression of Cul3 stimulates the accumulation of cyclin E-ubiquitin conjugates in a phosphorylation-independent manner, thereby demonstrating a critical link between Cul3 and the cellular cyclin E destruction machinery. Cul3 is likely to be required for destruction of cyclin E. However, it seems unlikely that the development defects are solely due to cyclin E accumulation, as Cul3 complexes will likely regulate the levels of other proteins in addition to cyclin E. Human Cul3 is a member of the Cullin family of E3 ubiquitin-protein ligases. Cul3 was found to bind to cyclin E but not to cyclin E-cdk2 complexes in mammalian cells, and that overexpression of Cul3 increased ubiquitination of cyclin E but not other cyclins. The SCF pathway, which targets cyclin for ubiquitination on the basis of their phosphorylation state, and the Cul3 pathway, which selects cyclin E for ubiquitination on the basis of its assembly into cdk complexes, may be a complementary way to control cyclin abundance.\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nImmunoprecipitation: 5-10ug/per IP reaction \nWestern Blot: 1-5ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C7949-21

Size

100ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Winston, J., et al., Genes and Dev. 13: 2,751-2,757, 1999. 2. Singer, J., et al., Genes and Dev. 13: 2,375-2,387, 1999.

Additional Info

Reacts with the ~89kD human Cullin 3 protein. Reactivity has been con?rmed with HeLa, NIH 3T3 and 293T cells. Species sequence homology: mouse Cullin 3. Does not cross-react with other species