Cullin proteins assemble a large number of RING E3 ubiquitin ligases and regulate various physiological processes (1). Cullin 3-based E3 ligase (CUL3) forms a catalytically inactive BTB-CUL3-Rbx1 (BCR) ubiquitin ligase, which becomes functional upon covalent attachment of the ubiquitin Nedd8 (neural precursor cell-expressed, developmentally downregulated 8) near the C terminus of CUL3 (2). CUL3 plays an essential role in both axonal arborization and proper elaboration of dendrites and may require neddylation for its proper function (3). The CUL3 pathway, which selects cyclin E for ubiquitination on the basis of its assembly into CDK complexes, may be one way to control cyclin abundance (4). In a complex with the substrate-specific adaptors KLHL9 and KLHL13, CUL3 is required for correct chromosome alignment in metaphase, proper midzone and midbody formation, and completion of cytokinesis (5).
Applications:
Suitable for use inFlow Cytometry, Western Blot, Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Western Blot: 1:20,000-100,000
Immunoprecipitation: 1:40
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
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