Cullin 3 (NT) (CUL-3)

CUL-3 is a member of the family of human cullin genes (CUL-1,-2,-3,-4a,-4b, and-5), homologous to the S. cerevisiae cdc53 gene. Cullin-3 is proposed to be a part of E3 protein-ubiqutinnn ligase. It forms a tight complex with cyclin E and targets it for ubiquitination, which leads to its degradation by protesomes. Cyclin E is an activator of cdk2, which leads the cell to S phase. Cul-3-/-mice show increased accumulation of Cyclin E protein and disruption of normal cell cycle regulation in vivo. Thus Cullin-3 is important in the regulation of S phase of cell cycle. \n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:2000-1:10,000\nWestern blot: 1:500-1:1,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7949-20F

Size

100ul

Applications

ELISA, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid, 0.01% sodium azide.

P Type

Pab

Purity

Serum

References

1. Furukawa, M., et al., (2003), \"Targeting of ubiquitination by BTB-Cullin3-Roc1 ubiquitin ligases\", Nat. Cell Biol., 5, 1001-1007. 2. Kipreos, E. T., et al., (1996), \"cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family\", Cell, 85 (6), 829-839. 3. Michel, J.J., et al., (1998), \"Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A\", Cell Growth Differ., 9 (6), 435-449. 4. Ohta, T., et al., (1999), \"ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity\", Mol. Cell., 3 (4), 535-541.5. Mathias, N., et al., (1996), \"Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins\", Mol. Cell Biol., 16:12, 6634-6643.

Additional Info

Recognizes human Cullin 3 at ~88.9kD.