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Cullin 3 (NT) (CUL-3)

Cullin 3 (NT) (CUL-3)

Cat no: C7949-20F

Supplier: United States Biological
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CUL-3 is a member of the family of human cullin genes (CUL-1,-2,-3,-4a,-4b, and-5), homologous to the S. cerevisiae cdc53 gene. Cullin-3 is proposed to be a part of E3 protein-ubiqutinnn ligase. It forms a tight complex with cyclin E and targets it for ubiquitination, which leads to its degradation by protesomes. Cyclin E is an activator of cdk2, which leads the cell to S phase. Cul-3-/-mice show increased accumulation of Cyclin E protein and disruption of normal cell cycle regulation in vivo. Thus Cullin-3 is important in the regulation of S phase of cell cycle. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 1:2000-1:10,000 Western blot: 1:500-1:1,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C7949-20F
Reactivities: Human
Hosts: Rabbit
Applications: ELISA, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid, 0.01% sodium azide.
P type: Pab
Purity: Serum
References: 1. Furukawa, M., et al., (2003), "Targeting of ubiquitination by BTB-Cullin3-Roc1 ubiquitin ligases", Nat. Cell Biol., 5, 1001-1007. 2. Kipreos, E. T., et al., (1996), "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family", Cell, 85 (6), 829-839. 3. Michel, J.J., et al., (1998), "Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A", Cell Growth Differ., 9 (6), 435-449. 4. Ohta, T., et al., (1999), "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity", Mol. Cell., 3 (4), 535-541.5. Mathias, N., et al., (1996), "Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins", Mol. Cell Biol., 16:12, 6634-6643.
Additional info: Recognizes human Cullin 3 at ~88.9kD.

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