Custom Monoclonal Antibodies (Standard Protocol, 120 Days)

Monoclonal Antibody Production \nTotal Time: ~4 months. \n\n* Free peptide designing\n* Immunization of 5 Balb/c mice\n* Fusion of spleen cells with SP2/0 myeloma cells.\n* ELISA screening for up to 3,000 clones.\n* Free Western blot screening for protein antigen\n* Guaranteed IgG isotype\n* Progress Tracking\n* ELISA positive guaranteed or your money back. \n \nPhase I \n*Immunization and boosts of 5 Balb/c mice with standard immunization protocol.\n*ELISA evaluation of titer prior to selection for fusion.\n*Approximately 3-4mg protein immunogen or 2mg conjugated peptide immunogen and 0.5mg free peptide are required. \n\nPhase II \n*Once an acceptable titer is obtained, hybridoma fusion will be done using splenocytes from mouse with the best titer and myeloma cells.\n*Supernatants from the growing hybridoma wells will be screened by ELISA. 10 clones will be sent to customer's lab for evaluation.\n\nPhase III \n*2 of these 10 clones selected by the customer will be subcloned by limiting dilution, isotyped and expanded.\n\nAdditional Services:\nPeptide synthesis and conjugation \nPeptide synthesis without conjugation\nPeptide conjugation\nAdditional clones (>10) in Phase II\nNegative screening\nExtra screening with IHC\nAdditional clones (>2) in Phase III\nCell-line adaptation to serum free medium\nCell banking and store in 10 ampules after first two months\nAscites production\nAntibody purification by Protein G or ammonium persulfate for IgM (10 mice minimum)\nAntibody culture in bioreactor\nAntibody culture in flask\nAntibody purification by protein G from one liter of medium\nAntibody purification by protein G from ascites (20mg)\nAntibody epitope mapping by peptide library\nAntibody conjugation with FITC, HRP, or Biotin\n\nClient retains all property rights to selected hybridomas and monoclonal antibodies developed in their project. Each project is conducted with complete confidentiality.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts