Custom Polyclonal Antibodies, Goat

USDA-Registered Polyclonal Production:\nUnited States Biological is known for more than the thousands of antibodies offered in our catalog. Our antibodies are produced in state of the art facilities in the US.\n\nWe maintain current USDA certification as a qualified animal research facility, and comply with the Animal Welfare Act. We follow guidelines set forth by the National Institute of Health (NIH) and have established an Institutional Animal Care and Use Commitee (IACUC) to review all production protocols prior, during and after all client programs.\n\nA typical standard protocol includes animal purchase, housing and care, all immunizations, bleeds and exsanguination as per our standard protocol formats. Your project may require different specifications and, therefore, prices will vary. Our costs are quite reasonable. Although we will conduct single animal programs, the additional costs to add a second animal can produce a significantly greater yield and likelihood for success.\n\nStandard Goat Protocol:\nThe schedule for goats is the same as for rabbits, but the immunogen dose is higher. One goat receives the dose of two rabbits. Goats take a little longer to develop titer than rabbits, so they are probably not suited for a rapid antibody development program.\n\nWe would take some pre-immune serum, and immunize with CFA to start, and immunize again at week 3 and week 6, with the first bleed taken at week 7.\n\nAdditional immunizations are done every four weeks, with bleeds taken one week after the immunization. Standard protocol pricing includes animal purchase, housing and care\nup to 56 days, all immunizations, bleeds and exsanguination as per the following schedule:\n\nPre-Peptide Consultation if necessary\nPeptide Production\nPeptide Conjugation\n\nDay 0 Pre-immunization bleed\n Antigen Injection (s.c and i.m; CFA; 400ug)\nDay 21 Antigen Injection (IFA; 200ug)\nDay 42 Antigen Injection (IFA; 200ug)\nDay 49 First Bleed (20-25ml/goat)\n ELISA\nDay 49 Antigen Injection (IFA; 200ug)\nDay 56 Final Production Bleed (~40-50ml/goat)\n ELISA\n Return of antisera and peptides\n\nContinuation (extra cost):\nDay 63 Antigen Injection (IFA; 200ug)\nDay 77 Third Bleed (20-25ml/goat)\n\nAntigen Requirements:\nPer Animal Minimum Recommended\nChickens 250ug 1mg\nRabbits 500ug 2mg\nGoats 2.5mg 5mg\nSheep 2.5mg 5mg\n\nThe amount of antigen required varies from a mininum as shown for the\nstandard protocol to a maximum when additional injections might be\nneeded to boost antibody production or to continue a maintenance\nprogram longer than the stated time. Affinity columns will also\nrequire additional antigen.\n\nImportant Note:\nStandard projects: All projects will automatically terminate 1 week after the expiration of the standard protocol unless a written request is made for the extension and additional charges are paid @$295/goat/month. This can be in the form of a Purchase Order. Please communicate with us in writing fax/email to avoid any confusion and make sure instruction are properly followed.\n\nAntibody Quotation Worksheet: \nFor a quotation, please supply the following information:\n\n1. Antigen name or peptide sequence\n\n2. Type of protein\no Natural\no Recombinant\no Synthetic \no Purity ___%\no MW. ___\n\n3. Conjugation\no BSA\no KLH\no Ovalbumin\no Thyroglobulin\n\n4. Number of animals required\n\n5. Immunization protocol\no Customer Supplied\no United States Biological Supplied\n\nImportant Note:\nStandard projects: All projects will automatically terminate 1 week after the expiration of the standard protocol unless a written request is made for the extension and additional charges are paid @$295/goat/month. This can be in the form of a Purchase Order. Please communicate with us in writing fax/email to avoid any confusion and make sure instruction are properly followed.

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