CX3CR1 (Beta Chemokine Receptor-like 1, CCRL1, Chemokine C-X3-C Motif Receptor 1, CX3C Chemokine Receptor 1, C-X3-C CKR-1, CX3C CKR-1, CMK-BRL-1, CMKBLR1, CMKDR1, Fractalkine Receptor, GPR13, GPRV28, V28)

Fractalkine, also termed neurotactin, is a membrane-bound CX3C chemokine1. human CX3CR1, also named V28, was first cloned from human genomic DNA libraries. A G-protein-coupled seven-transmembrane domain receptor, was recently identified to serve as fractalkine receptor. Human CX3CR1 has been shown to mediate both the adhesive and migratory functions of fractalkine. Fractalkine and CX3CR1 represent new types of leukocyte trafficking regulators.\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:2000\nImmunoprecipitation: 1:300-1:800\nImmunohistochemistry: 1:100-1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C8299-01B

Size

200ug

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid in PBS with 0.1% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

References

1. Bazan, J.F. et al. (1997) A new class of membrane-bound chemokine with a CX3C motif. Nature 385:640-644 2. Harrison, J.K. et al. (1994) cDNA cloning of a G-protein-coupled receptor expressed in rat spinal cord and brain related to chemokine receptors. Neurosci Lett 169:85-89 3. Imai, T. et al. (1997) Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion. Cell 91:521-530 4. Combadiere, C. et al. (1998) Identification of CX3CR1. A chemotactic receptor for the human CX3C chemokine fractalkine and a fusion coreceptor for HIV. J Biol Chem 273(37):23799-23804.

Additional Info

Reacts with recombinant and natural human and rat CX3CR1.