CX3CR1 (Beta Chemokine Receptor-like 1, CCRL1, Chemokine C-X3-C Motif Receptor 1, CX3C Chemokine Receptor 1, C-X3-C CKR-1, CX3C CKR-1, CMK-BRL-1, CMKBLR1, CMKDR1, Fractalkine Receptor, GPR13, GPRV28, V28) (PE)

CX3CR1 is a 40kD seven transmembrane chemokine receptor that is expressed on several T cell subsets, monocytes, macrophages, microglia and epithelial cells. CX3CR1 binding to membrane bound or soluble CX3CR1/Fractalkine promotes inflammatory responses by inducing monocyte adhesion to endothelial cells and macrophage activation. CX3CR1 polymorphisms are associated with the development of chronic inflammatory disorders. Alternately spliced isoforms have extended N-terminal extracellular regions that increase the potency of CX3CR1 as a fusion coreceptor for HIV-1. Human CX3CR1 shares 82% amino acid sequence identity with mouse and rat CX3CR1.\n\nApplications:\nSuitable for Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 10ul labels 1x10e6. Validated using human peripheral blood monocytes.\nOptimal dilutions to be determined by the researcher.\n\nFlow Cytometry Protocol:\n1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.\n2. Add 10ul of C8299-01F to (same/less than)1x10e6 cells and incubate for 30 minutes at RT.\n3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.\n4. Resuspended cells in F4568-10 for flow cytometry analysis. As a control for this analysis, cells in a separate tube should be treated with PE-labeled mouse IgG1 antibody.\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Stable for 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

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