CXCL10/IP-10 BioAssay(TM) ELISA Kit, Mouse

Sandwich ELISA quantitative detection kit for Mouse CXCL10/IP-10 in cell culture supernates, serum and plasma (heparin).\n\nChemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10kD interferon-gamma-induced protein (g-IP10 or IP-10). CXCL10 is secreted by several cell types in response to IFN-g. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers.This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3. \n\nAssay Range:\n31.2pg/ml-2000pg/ml\n\nSensitivity:\n< 1 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP17515\n\nEntrez Gene ID:\n15945\n\nKit Components:\n96-well plate pre-coated with anti-CXCL10/IP-10, Mouse\nLyophilized recombinant CXCL10/IP-10, Mouse standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143791

Size

96Tests

References

1. Luster et al. Gamma-interferon transcriptionally regulates an early-response gene containing homology to platelet proteins. Nature 315: 672-676, 1985.\n2. Dufour et al. IFN-gamma-inducible protein 10 (IP-10; CXCL10) -deficient mice reveal a role for IP-10 in effector T cell generation and trafficking. J. Immun. 168:, 2002.\n3. Angiolillo et al. Human interferon-inducible protein 10 is a potent inhibitor of angiogenesis in vivo. J. Exp. Med. 182: 155-162, 1995.\n4. Angiolillo, A. L.; Sgadari, C.; Taub, D. D.; Liao, F.; Farber, J. M.; Maheshwari, S.; Kleinman, H. K.; Reaman, G. H.; Tosato, G. : Human interferon-inducible protein 10 is a potent inhibitor of angiogenesis in vivo. J. Exp. Med. 182: 155-162, 1995.\n5. O'Donovan et al. Physical mapping of the CXC chemokine locus on human chromosome 4. Cytogenet. Cell Genet. 84: 39-42, 1999.\n6. Zhang, R.; Zhang, H.; Zhu, W.; Pardee, A. B.; Coffey, R. J., Jr.; Liang, P. : Mob-1, a Ras target gene, is overexpressed in colorectal cancer. Oncogene 14: 1607-1610, 1997.\n7. Booth et al. The CXCR3 binding chemokine IP-10/CXCL10: structure and receptor interactions. Biochemistry 41:, 2002.\n