CXCL3 (C-X-C Motif Chemokine 3, Dendritic Cell Inflammatory Protein 1, Dcip1, Gm1960)

CXCL3 is also known as MIP-2B, or DCIP-1 in mouse, CINC2 in rat, and GRO-G in humans. It is an 8kD proinflammatory member of the CXC subfamily of heparin-binding chemokines, also called alpha chemokines. The Glu-Leu-Arg (ELR) motif near the CXCL3 N-terminus confers angiogenic properties and distinguishes it from interferon-inducible ELR- CXC chemokines, which are angiostatic. ELR+ and ELR- chemokines use CXCR2 and CXCR3 receptors, respectively. Mature mouse CXCL3 shares 88% and 57% aa sequence identity with rat and human CXCL3, respectively. Among mouse ELR+ chemokines, it shares 82% aa sequence identity with CXCL2/GRO-B/MIP-2 and 34%-58% with CXCL1/GRO-A/KC, CXCL5/ENA-78 and CXCL7/NAP-2. Due to their similar sequence and activity, CXCL2 and CXCL3 are sometimes referred to collectively as CXCL2/3, but are separate gene products. Mouse CXCL3 expression is induced in macrophages and early in maturation of DC by bacterial products such as lipopolysaccharides, and other inflammatory mediators. It is chemotactic for CXCR2-expressing neutrophils, helping to recruit them to areas of inflammation. ELR+ chemokines also elicit endothelial cell chemotaxis, stimulating angiogenesis and playing a role in tumor development. ELR+ chemokines upregulated by ischemia play a role in ischemia-reperfusion injury. A decoy receptor, DARC competes with CXCR2 for ELR+ chemokine binding, thus downregulating their effect. Neutrophil influx may also be downregulated by MMP-12, which has been found to inactivate CXCL3 and other ELR+ chemokines by cleaving them at the ELR site. Over aa28-100, mouse CXCL3 shares 87.8% aa identity with rat CINC2.\n\nApplications:\nSuitable for use in Western Blot and Neutralization. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1ug/ml\nNeutralization: 0.5-3ug/ml in the presence of 100ng/m recombinant mouse CXCL3.\nOptimal dilutions to be determined by the researcher.\n\nEndotoxin: ~0.1EU/1ug (LAL)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C8297-98B

Size

100ug

Applications

WB

Hosts

Sheep

Reactivities

Mouse

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Nolan, K. F. et al. (2004) J. Immunol. 172:2201. 2. Modi, W. S. and T. Yoshimura (1999) Mol. Biol. Evol. 16:180. 3. Vandercappellen, J. et al. (2008) Cancer Lett. 267:226. 4. Strieter, R.M. et al. (2005) Cytokine Growth Factor Rev. 16:593. 5. Nesmelova, I. V. et al. (2008) J. Biol. Chem. 283:24155. 6. Maheshwari, A. et al. (2004) Fetal Pediatr. Pathol. 23:145. 7. Furuichi, K. et al. (2008) Front. Biosci. 13:4021. 8. Takano, K. and H. Nakagawa (2001) Inflamm. Res. 50:503. 9. Horton, L. W. et al. (2007) Cancer Res. 67:9791.

Additional Info

Recognizes mouse CXCL3. Species sequence homology: Recombinant rat (50%).