CXCR3 (PE) (CD183)

CXCR3 is a cell surface chemokine receptor that belongs to the family of G protein linked, seven transmembrane domain containing receptors. With the cloning of this molecule, it was identified as the specific receptor for two CXC chemokines: gamma interferon induced protein and monokine induced by interferon gamma. The CXCR3 receptors appear to have affinity for CXC chemokines that do not contain the conserved ELR motif at their amino terminus and as a result IL-8 and platelet factor 4 do not bind to this receptor. It shares approx. 40% monology with the CXCR1 and CXCR2 receptors and approx 35% homology with the first five CCR receptors. In spite of the fact that the two ligands for CXCR3 are members of the a-chemokine family, which has historically linked with chemotactic activity of neutrophils, IP10 and MIG, are the first molecules to target lymphocytes. This is consistent with the mRNA expression pattern of CXCR3 molecules, where it was found on activated T, B, and NK cells but not on monocytes, resting T cells, or neotrophils. Of clinical interst is the demonstration of CXC3 expression on cells from B cell chronic lymphocytic leukimeias and splenic marginal zone lymphomas.\n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution: \nFlow Cytometry: 10ul/10e5 cells to detect human CXCR3. \nOptimal dilution to be determined by researcher.\n\nHybridoma: \nBalb/c mouse inoculated with hCXCR3 transfected NS0 mouse myeloma cells. \n\nSource: Ascites fluid\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

C8349-02

Size

1ml

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Loetscher, M., et al., 1996, J Exp. Med. 184:963. 2. Farber, J.M., 1997, J. Leukoc. Biol. 61:246. 3. Liao, F., et al., 1995, J Exp Medicine 182:1301. 4. Qin, S., et al., 1998, J. clin Invest. 101:746. 5. Jones, D., et al., 2000, Blood 95:627.

Additional Info

Recognizes recombinant, human CXCR3. Reacts specifically with CXCR3 transfectants. Does not recognizes the parental cell line using FACS analysis. Does not crossreact with CXCR1, CXCR2 and CXCR4 transfectants.