CXCR4 (Fusin, LESTR, HUMSTR, C-X-C Chemokine Receptor 4, CD184)

CXCR4 (fusin) is a members in the seven-transmembrane domain G-protein-coupled chemokine receptor family. The full-length cDNA was isolated from a human spleen cDNA library1. Its ligand is chemokine stroma-derived factor (SDF). CXCR4 has been shown to be the co-receptor for HIV-1 binding to CD4 through the viral envelope glycoprotein gp1202. In other cases, CXCR4 can function as the only receptor for the interaction of HIV-2 with CD4 and host cells3. A mouse monoclonal antibody (12G5) to CXCR4 has been shown to inhibit HIV infectivity and HIV-induced syncytium.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:2000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C8350-06B

Size

200ug

Applications

WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as a lyophilized powder in 0.1% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

References

1. Federsppiel, B. et al. (1993) Molecular cloning of the cDNA and chromosomal localization of the gene for a putative seven-transmembrane segment (7-TMS) receptor isolated form human spleen. Genomics 16 (3): 707-12 2. Nagasawa, T. et al. (1998) A novel CXC chemokine PBSF/SDF-1 and its receptor CXCR4: their functions in development, hematopoiesis and HIV. Semin Immunol 10 (3): 179-85 3. Mcknight, A. et al. (1997) Inhibition of Human Immunodeficiency Virus Fusion by a Monoclonal Antibody to a Coreceptor (CXCR4) Is both Cell Type and Virus Strain Dependent. J. Virol., 71 (2): 1692-1696.

Additional Info

Reacts with rat and mouse CXCR4.