Viral entry into target cells is mediated through a complex interaction between the viral envelope protein and specific cellular receptors. The CD4 glycoprotein is the primary receptor for human HIV types 1 and 2. However, one or more cellular coreceptors are also needed for viral entry. CXCR4 has been identified as a co-receptor for HIV type 1. T lymphocytes and macrophages in both lymphoid and non-lymphoid tissues are the major cell populations expressing CXCR4. CXCR4, also known as Chemokine (C-X-C motif) Receptor 4 or CD184, is a receptor for the CXC chemokine stromal cellderived factor-1 (SDF-1) and is involved in cardiogenesis, blood vessel formation, and cerebellular development. CXCR4 has been reported to be ubiquitously expressed in blood and tissue cells.
Applications:
Suitable for use in Immunohistochemistry, Immunofluorescence and Flow Cytometry. Other applications have not been tested.
Recommended Dilution:
Immunohistochemistry: 10ug/ml
Immunofluorescence: 10ug/ml. Do not use cells fixed in acetone or methanol. 3% paraformaldehyde has been shown to give optimal results.
Flow Cytometry: 2-5ug/ml
Optimal dilutions to be determined by the researcher.
Positive Controls:
Human lymphoid cell lines, human liver and brain tissues.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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