CXCR4-Lo (Fusin, LESTR, HUMSTR)

Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors (1). Among them, CXCR4 is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells (2,3). CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2 (4,5). The a- chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1 (6,7). CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells (8). CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types (2). Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells (2,5,10). The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites (10,11). This antibody is specific for the longer isoform of CXCR4.\n \nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 5

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SPECIFICATIONS

Catalog Number

C8350-02A

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquidin PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified.

Isotype

IgG

References

1. Dimitrov DS. Cell 1997; 91:721-30. 2. Feng Y et al. Science 1996; 272:872-7. 3. Berson JF et al. J. Virol. 1996; 70:6288-95. 4. Doranz BJ et al. Cell 1996; 85:1149-58. 5. Endres MJ et al. Cell 1996; 87:745-56. 6. Bleul CC et al. Nature 1996; 382:829-3. 7. Oberlin E et al. Nature 1996; 382:833-5. 8. Lapham CK et al. Science 1996; 274:602-5. 9. Leoetscher M et al. J. Biol. Chem. 1994; 269:232-7. 10. Brelot A et al. J. Virol. 1997; 71:4744-51. 11. Lu Z et al. Proc. Natl. Acad. Sci. USA 1997; 94:6426- 31.

Additional Info

Recognizes the longer isoform of human CXCR4.