CXCR7 (C-X-C Chemokine Receptor 7, Chemokine Orphan Receptor 1, RDC-1) (PE)

The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7. CXCR7 can bind with high-affinity to CXCL12/SDF-1 and CXCL11/I-TAC. It is also a co-receptor for several HIV and SIV strains. In their N-termini and extracellular loops 1, 2, and 3, human and mouse CXCR7 share 84%, 100%, 96% and 86% amino acid sequence identity, respectively. Reports of mRNA levels and/or protein expression indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. In contrast, based on ligand binding analysis and receptor level (as assessed using C8376-26), surface expression of CXCR7 was reported to be restricted to tumor cells, activated endothelial cells, fetal liver cells, and few other cell types. The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes. \n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: Neat. Use 10ul to label 10e6 cells.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Stable for at least 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

C8376-25B

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.5% BSA, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

Additional Info

Recognizes human CXCR7. Reacts specifically as detected by Flow Cytometry with five distinct human CXCR7 transfectants, but not their respective parental lines. Also reacts with monocytes expression of CXCR7. Does not react with MCF-7 cells by Flow Cytometry, which have been reported to have surface-expressing CXCR7 using C8376-26. Due to the conflicting reports published, use of monoclonal C8376-25 may result in an underestimation of CXCR7 expression on certain cell types.