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Products Cyclic AMP EIA Kit (without Acetic Anhydride)
| Catalogue number: | 581002 |
| Weight: | 0 |
| Form: | 96 Well |
| P type: | Assay Kits|Cyclic Nucleotides |
| Shipping temp: | -20 |
| Storage temp: | -20 |
| Additional info: | cAMP is a ubiquitous cellular second messenger that is a critical component of a signal transduction pathway linking membrane receptors and their ligands to the activation of internal cellular enzymatic activity and gene expression. cAMP is synthesized from ATP by membrane-bound adenylate cyclase and activates or inhibits various enzymes or cascade of enzymes by promoting their phosphorylation or dephosphorylation. Cayman's cAMP assay is a competitive EIA that permits cAMP measurements within the standard curve range of 0.08-10 pmol/ml, typically with a limit of quantification of 0.1 pmol/ml (80% B/B0) for acetylated cAMP. Non-acetylated cAMP can be assayed with an IC50 value (50% B/B0) of approximately 20 pmol/ml and a detection limit (80% B/B0) of 3 pmol/ml. |
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