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Cyclin B1 (G2/Mitotic-specific Cyclin-B1, Ccnb1, Ccn-2, Ccnb1-rs13, Cycb, Cycb1)

Cyclin B1 (G2/Mitotic-specific Cyclin-B1, Ccnb1, Ccn-2, Ccnb1-rs13, Cycb, Cycb1)

Cat no: C8453-04F

Supplier: United States Biological
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Cyclin B1 belongs to the cyclin family, Cyclin AB subfamily, and predicted molecular weight 48kD. Cyclin B1 is expressed ubiquitously in all eukaryotes and the level of cyclin B1 was minimal in G, began to rise in S phase, and is primarily cytoplasmic. Cyclin B1 moves into the nucleus just at the start of mitosis and associates with condensed chromosomes and the mitotic spindle. The level of cyclin B1 peaked at the G/M transition. Cyclin B1 is essential for the control of the cell cycle at the G2/M transition. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 2.5-5ug/5ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C8453-04F
Reactivities: Human, Mouse
Hosts: Mouse
Applications: Immunofluorescence, Immunohistochemistry, Western Blot
Size: 25ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Morgan DO, et al.1995. Nature 374:131.
Additional info: Recognizes human Cyclin B1. Species Crossreactivity: mouse.

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