Cyclin D1 (bcl 1)

Cyclin D1 gene is identical with the BCL-1 and the PRAD-1 oncogene and is part of the chromosome 11q13 amplicon which is amplified in several common tumor types like breast, lung, esophagus, urinary bladder and head and neck carcinoma. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. About 50-70% of mantle cell lymphomas (MCL) contain a t (11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. Cyclins were originally identified as proteins whose levels fluctuated during the cell cycle. Subsequent studies have shown the cyclins control entry into different phases of the cycle cell by regulating the activity of cyclin-dependent kinases (CDKs). Mitotic or G2 cyclins regulate entry into M phase, whereas G1 cyclins regulate entry into S phase. Cyclin D1, a G1 cyclin, is essential for control at the G1/S transition (START in yeast), as it regulates the activity of CDK4 and CDK6 protein kinases. Translocation of the gene encoding cyclin D1, the bcl-1 oncogene, is associated with human B-lymphocytic malignancy, suggesting that alteration of cell cycle progression may be oncogenic.\n\nApplications: Biological Blockade (Ab should be ordered without azide), Flow Cytometry, Immunofluorescence, Immunoprecipitation and Western Blot.\nOther applications not tested.\n\nRecommended Dilution:\nImmunoprecipitation: Use Protein A; 2ug antibody/mg protein lysate (co-precipitates cdk4)\nWestern Blot: 1-2ug/ml for 2hrs at RT \nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMAD109 cells. Mantle cell lymphomas and breast carcinoma.\n\nStorage and Stability:\nMay be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.\nNote: We do not recommended adding glycerol as a cryopreservative.

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SPECIFICATIONS

Catalog Number

C8454-02

Size

500ul

Applications

FC, IF, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can, NHP

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. Also available without BSA and azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. Lukas J, et. al. Oncogene, 1994, 9(3):707-18. 2. Gillett C, et. al. Cancer Research, 1994, 54(7):1812-7.\n3. Bartkova J, et. al. Journal of Pathology, 1994, 172(3):237-45. 4. Bartkova J, et. al. Oncogene, 1995, 10(4):775-8.

Additional Info

Recognizes Cyclin D1 at 36kD. \nSpecies Crossreactivity: Human, monkey, mouse, rat and canine.