Cyclin D1 (bcl 1)

Cyclin D1or PRAD-1 or bcl-1 is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. It is a putative protooncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL).\n\nEpitope: C-terminus.\n\nApplications:\nImmunofluorescence\nImmunoprecipitation: (Use Protein A) (Ab 10ug/mg protein lysate)\nWestern Blot: (Ab 5ug/ml for 2hrs at RT)\nImmunohistology (Formalin/paraffin): (Ab 10-20ug/ml for 30 min at RT) Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\n\nPositive Control: MAD109 cells. Breast carcinoma or mantle cell lymphoma.\n\nCellular Localization: Nuclear.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C8454-03A

Size

250ul

Applications

ELISA, IF, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Lukas J, et. al. Molecular and Cellular Biology, 1995, 15(5):2600-11.\n2. Lukas J; et al. Oncogene, 1995,10:2125-34.\n3. Lukas J; etal. Oncogene, 1994, 9(3):707-18.\nAdditional Suggested References:\n1. Baldin V; Lukas J; Marcote MJ; Pagano M; Draetta G. Cyclin D1 is a nuclear protein required\nfor cell cycle progression in G1. Genes and Development, 1993, 7(5):812-21.\n2. Bartkova J; Lukas J; Muller H; Lutzhoft D; Strauss M; Bartek J. Cyclin D1 protein expression\nand function in human breast cancer. International Journal of Cancer, 1994, 57(3):353-61.\n3. Bartkova J; Lukas J; Strauss M; Bartek J. Cell cycle-related variation and tissue-restricted\nexpression of human cyclin D1 protein. Journal of Pathology, 1994, 172(3):237-45.\n4. Bartkova J; Lukas J; Strauss M; Bartek J. The PRAD-1/cyclin D1 oncogene product accumulates\naberrantly in a subset of colorectal carcinomas. International Journal of Cancer, 1994, 58(4):568-73.\n5. Bartkova J; Lukas J; Strauss M; Bartek J. Cyclin D1 oncoprotein aberrantly accumulates in\nmalignancies of diverse histogenesis. Oncogene, 1995, 10(4):775-8.\n6. Bartkova J; Lukas J; Muller H; Strauss M; Gusterson B; Bartek J. Abnormal patterns of D-type\ncyclin expression and G1 regulation in human head and neck cancer. Cancer Research, 1995, 55:949-56.\n7. Bates S; Bonetta L; MacAllan D; Parry D; Holder A; Dickson C; Peters G. CDK6 (PLSTIRE)\nand CDK4 (PSK-J3) are a distinct subset of the cyclin-dependent kinases that associate with cyclin\nD1. Oncogene, 1994, 9(1):71-9.\n8. Bates S; Peters G. Cyclin D1 as a cellular protooncogene. Seminars in Cancer Biol, 1995, 6:73-82.\n9. Dowdy SF; Hinds PW; Louie K; Reed SI; Arnold A; Weinberg RA. Physical interaction of the\nretinoblastoma protein with human D cyclins. Cell, 1993, 73(3):499-511.\n10. Ewen ME; Sluss HK; Sherr CJ; Matsushime H; Kato J; Livingston DM. Functional interactions of\nthe retinoblastoma protein with mammalian D-type cyclins. Cell, 1993, 73(3):487-97.\n11. Gillett C; Fantl V; Smith R; Fisher C; Bartek J; Dickson C; Barnes D; Peters G. Amplification and\noverexpression of cyclin D1 in breast cancer detected by immunohistochemical staining. Cancer Research,\n1994, 54(7):1812-7.\n12. Hanna Z; Jankowski M; Tremblay P; Jiang X; Milatovich A; Francke U; Jolicoeur P. The Vin-1\ngene, identified by provirus insertional mutagenesis, is the cyclin D2. Oncogene, 1993, 8(6):1661-6.\n13. Hunter T; Pines J. Cyclins and cancer. II: Cyclin D and CDK inhibitors come of age [see comments].\nCell, 1994 Nov 18, 79(4):573-82.\n14. Jiang W; Kahn SM; Zhou P; Zhang YJ; Cacace AM; Infante AS; Doi S; Santella RM; Weinstein IB.\nOverexpression of cyclin D1 in rat fibroblasts causes abnormalities in growth control, cell cycle\nprogression and gene expression. Oncogene, 1993, 8(12):3447-57.\n15. Jiang W; Zhang YJ; Kahn SM; Hollstein MC; Santella RM; Lu SH; Harris CC; Montesano R;\nWeinstein IB. Altered expression of the cyclin D1 and retinoblastoma genes in human esophageal\ncancer. Proceedings of the National Academy of Sciences of the United States of America, 1993,\n90(19):9026-30.\n16. Inaba T; Matsushime H; Valentine M; Roussel MF; Sherr CJ; Look AT. Genomic organization,\nchromosomal localization, and independent expression of human cyclin D genes. Genomics,\n1992, 13(3):565-74.\n17. Kato J; Matsushime H; Hiebert SW; Ewen ME; Sherr CJ. Direct binding of cyclin D to the\nretinoblastoma gene product (pRb) and pRb phosphorylation by the cyclin D-dependent kinase\nCDK4. Genes and Development, 1993, 7(3):331-42.\n18. Leach FS; Elledge SJ; Sherr CJ; Willson JK; Markowitz S; Kinzler KW; Vogelstein B.\nAmplification of cyclin genes in colorectal carcinomas. Cancer Research, 1993, 53(9):1986-9.\nLew DJ; Dulic V; Reed SI. Isolation of three novel human cyclins by rescue of G1 cyclin (Cln)

Additional Info

Recognizes Cyclin D1. Species Crossreactivity: Human, Mouse and Rat.