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Cyclin D1

Cat no: C8454-01B

Cyclin D1

Cyclin D1 is a nuclear protein (36kD ). D type cyclins are predominantly expressed in the G1 phase of the cell cycle. Cyclin D1 study is of interest in various forms of malignancy and over expression has been linked to poor prognosis.\n\nApplications:\nSuitable for use in Flow Cytometry, Immunohistochemistry (frozen and paraffin), ELISA, Immunoprecipitation and Western Blot. Other applications not tested. \n\nRecommended Dilution: \nWestern Blot: 1:2000\nFlow Cytometry: Neat; Use 5ul of the optimal working dilution to stain 10e6 cells in 100ul. Permeabilization is recommended prior to staining.\nImmunohistochemistry (Paraffin and frozen): 1:1000-1:10,000 Note: Paraffin sections require antigen retrieval using sodium citrate buffer, pH 6.0, heat treatment prior to staining.\nOptimal working dilutions to be determined by researcher.\n\nRecommended Positive Control Tissue: \nSmall intestine, colon.\n\nRecommended Secondary Reagents:\nFlow Cytometry:\nI1904-66A: IgG, F(ab

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SPECIFICATIONS

Catalog Number

C8454-01B

Size

100ug

Applications

ELISA, FC, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Rat

Form

Supplied as a liquid in Tris-glycine, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes the Cyclin D1 (36kD). Species Crossreactivity: Human and rat.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info
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