Cyclin D1 (G1/S-specific Cyclin-D1, CCND1, B-cell Lymphoma 1 Protein, BCL-1, BCL-1 Oncogene, PRAD1 Oncogene, BCL1, PRAD1)

Cyclin D1 gene is identical with the BCL-1 and the PRAD-1 oncogene and is part of the chromosome 11q13 amplicon which is amplified in several common tumor types like breast, lung, esophagus, urinary bladder and head and neck carcinoma. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. About 50-70% of mantle cell lymphomas (MCL) contain a t (11; 14)(q13; q32) translocation resulting in over-expression of Cyclin D1. Cyclins were originally identified as proteins whose levels fluctuated during the cell cycle. Subsequent studies have shown the cyclins control entry into different phases of the cycle cell by regulating the activity of cyclin-dependent kinases (CDKs). Mitotic or G2 cyclins regulate entry into M phase, whereas G1 cyclins regulate entry into S phase. Cyclin D1, a G1 cyclin, is essential for control at the G1/S transition (START in yeast), as it regulates the activity of CDK4 and CDK6 protein kinases. Translocation of the gene encoding Cyclin D1, the bcl-1 oncogene, is associated with human B-lymphocytic malignancy, suggesting that alteration of cell cycle progression may be oncogenic.\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 4ug/ml\nImmunohistochemistry (Formalin and paraffin): 8ug/ml\nImmunohistochemistry (Frozen): 8ug/ml on acetone fixed tissues\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C8454-02E

Size

100ug

Applications

ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1ml sterile PBS.

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG2a

References

1. Rosenberg, C.L., et al., Oncogene 6: 449-453 (1991). 2. Muller, H., et al., PNAS 91: 2945-2949 (1994). 3. Hinds, P.W., et al., PNAS 91: 709-713 (1994).

Additional Info

Recognizes human Cyclin D1. Species Crossreactivity: mouse and rat.