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Cyclin D1 (PRAD-1, bcl 1) (FITC)

Cat no: C8454-02B

Cyclin D1 (PRAD-1, bcl 1) (FITC)

Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk), which control transition at different specific phases of the cell cycle. In mammalian cells, Cyclin D1 plays a critical role in G1 to S transition. Cyclin D1 is a putative proto-oncogene.\n\nApplications: \nSuitable for use in Immunofluorescence and Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 0.5-1.0ug to stain 106 cells fixed in 75% ethanol. \nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nUnstimulated human PBMCs, T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, U-2-OS, HT29, Ramos, Jurkat, KNRK, C32, A431 cells.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

C8454-02B

Size

100ug

Applications

FC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can, NHP

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 15 mM sodium azide. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a,k

References

1. Darzynkiewicz, Z., et al. (1996) Cytometry 25: 1-13. 2. Bartkova, J., et al. (1996) Cancer Research 56: 5475-5483.

Additional Info

Recognizes human Cyclin D1 at ~36kD. Species Crossreactivity: Human, monkey, mouse, rat, and canine Cyclin D1. Other species were not tested.\n

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info
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