Cyclin D3 (CCNE 1, CCNE, Cyclin E1, Cyclin Es, Cyclin Et, G1/S specific cyclin E, CCND3)

Cyclin D3 is a member of Cyclin D family which mediates cell cycle progression through the G1 phase. Cyclin D family binds and activates cyclin-dependent kinases 4 and 6 CDK4, CDK6), which regulates the phosphorylation of retinoblastoma (Rb) protein, p107, and p130 (1). Cyclin D3, similar to cyclin D1 and D2, regulates the initial G1 to S transition by inhibiting Rb and activating E2F proteins (2). Cyclin D3 expression has been linked to inhibition of granulocyte differentiation and activation of various transcription factors, such as transcription factor 5 (3, 4). Overexpression of cyclin D3 is associated with poor clinical outcome in diffuse large B-cell lymphoma cases (5). \n\nApplications:\nSuitable for use inFlow Cytometry, Western Blot, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:5000 \nImmunocytochemistry: 1:100-250 \nFlow Cytometry: 1:30 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

C8454-17A

Size

100ul

Applications

FC, ICC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50 mM Tris-Glycine (pH 7.4), 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Sarruf D, et al. MCB, 25(22):9985-9995, 1995. 2. Dyson N, et al. Genes Dev. 12:2245-2262, 1998. \n3. Liu W, et al. Biochem. Biophys. Res. Commun. 321:954-960, 2004. 4. Kato JY, et al. PNAS 90:11513-11517, 1993. 5. Filipits M, et al. Clin. Can. Res. 8:729-733, 2002.

Additional Info

Recognizes human Cyclin D3.