Home  >  Products  >  Cyclin E
Cyclin E

Cyclin E

Cat no: C8455-25A

Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Read More on Supplier Website
Cyclin E is a regulatory subunit of cdk-2 and is thought to be rate limiting for the G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues, suggesting a post-transcriptional regulation of cyclin E. In vitro analyses indicated that these truncated variant isoforms of cyclin E are able to phosphorylate histone H1. Qualitative and quantitative alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers. Ab-1 is superb for biochemical applications including co-precipitation of cdk2. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:2000 Western Blot: 1:200-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C8455-25A
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: ELISA, Western Blot
Size: 100ul
Form: Supplied as a liquid, 0.01% sodium azide.
P type: Pab
Purity: Serum
References: 1. Koff, A., et al., (1991), "Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family", Cell, 66; 1217-1228. 2. Koff, A., et al., (1992), "Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle", Science, 257; 1689-1 694. 3. Claudio P.P., et al., (1996), "Functional analysis of pRb/p130 interaction with cyclins", Cancer Res., 56; 2003-2008.
Additional info: Recognizes human Cyclin E at ~45kD. Species Crossreactivity: rat, mouse.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave