Although cyclin E has an important physiological role in normal cell division, recent reports suggest this function is redundant, and its key functions occur as cells exit quiescence, and in endoreduplication.
Cyclin E is tightly regulated during normal cell cycles, but is often deregulated in carcinomas, lymphoma, and leukaemia.
Applications:
Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:2000
Western Blot: 1:200-1:500
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.