Cyclin E, p52

Cyclin E is a regulatory subunit of cdk-2 and controls G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues. Qualitative and quantitative alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml detects cyclin E (~52kD) in 20ug of human A431 lysate. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C8455-05 (0.5ug/ml). Proteins were visualized by using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. \nImmunoprecipitation: 4ug immunoprecipitated cyclin E from 500ug of human A431 RIPA cell lysate as confirmed by Western blot analysis of the immunoprecipitate using rabbit polyclonal anti-cyclin E.\nImmunocytochemistry: 10ug/ml detected cyclin E in 50% ethanol/50% acetic acid-fixed human A431 cells.\nOptimal dilutions to be determined by the researcher.\n\nPositive Antigen Control (Included): \nA0001-50 (Non-stimulated A431 cell lysate). \n\nI*Recommended secondary antibodies:\nI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo\nI1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo\n1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo\nI1904-23: IgG, X-Adsorbed (DTAF) Pab Gt xMo\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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