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Cyclin E1

Cyclin E1

Cat no: C8455-26E

Supplier: United States Biological
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Cyclin E1 is a protein (approx. 50 kD) expressed in elevated levels in human tumor cells. It controls the initiation of DNA synthesis by activating cdk2, and expression of cyclin E1 is upregulated by the E7 oncoprotein. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C8455-26E
Reactivities: Human
Hosts: Rabbit
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: Zariwala, M., Liu, J. and Xiong, Y. 1998 Oncogene 17: 2787-2798.
Additional info: Recognizes human Cyclin E1. Cyclin E1 expression is elevated in human tumor cells. Reacts with residues 43-52 [GSQPWDNNAVC] of Cyclin E1.

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