Cyclins are, as their name suggests, expressed at specific times during the cell cycle, and play a crucial role in the regulation of the cell cycle. Cyclins associate with cyclin-dependent kinases (cdks), and the association is absolutely required for cdk activity. At the conclusion of the relevant phase of the cell cycle, cyclins are rapidly ubiquitinated and degraded, thus returning cdks to their inactive state. Cyclin A associates with cdk2, and together with cyclin E is involved in the G1/S transition. Cyclin A/cdk2 remains active during S-phase. Cyclin B associates with cdk1/cdc2 to regulate the G2/M boundary. The D-cyclins bind cdk4 and cdk6, and result in Rb phosphorylation. The cyclin D1 gene was found in chronic B cell lymphocytic leukemia, and was designated Bcl-1 in a previously used nomenclature.
Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:20
Western Blot: 1:1000-1:2000
Immunocytochemistry: 1:100-1:250
Immunoprecipitation: 1:80
Optimal dilutions to be determined by the researcher.
Positive Control:
HeLa whole cell lysate.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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