Cyclin E1 Complex, Recombinant, Human (CCNE1, G1/S-specific Cyclin-E1, CCNE)

Cyclin E1 functions as a regulator of the cell cycle, promoting the G1 to S phase transition by binding to and activating the cyclin dependent kinase CDK2. The abundance of cyclin E1 fluctuates during a normal cell cycle and is controlled at the level of gene transcription and by ubiquitin dependent proteolysis. Non-complexed cyclin E1 is degraded by the Cul3 pathway, however when cyclin E1 is bound to CDK2 its ubiqutination is dependent on a positive phosphorylation status, which enables its recognition by SCFFbw7. The impact of cyclin E1 quantities on cell cycle regulation is reflected in the elevated levels expressed by many types of human tumours.\n\nSource: \nRecombinant corresponding to full length human Cyclin E1, fused to 6-His-tag at N-terminal c-Myc and full length human CDK2, co-expressed by baculovirus in Sf21 insect cells.\n\nMolecular Weight: \nCyclin E1: ~52kD, CDK2: ~34kD\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

029449

Size

10ug

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris/HCl pH7.5, 150mM sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.

Purity

~64% (SDS-PAGE, Coomassie blue staining)

References

1. Ohtsubo M. et al., Human Cyclin E, a Nuclear Protein Essential for the G1-to-S Phase Transition. Mol Cell Biol, 15: 2612-2624, 1995. 2. Won K-A. and Reed S.I. Activation of Cyclin E/CDK2 is Coupled to Site-Specific Autophosphorylation and Ubiquitin-Dependent Degradation of Cyclin E. The EMBO Journal, 15: 4182-4193, 1996. 3. Welcker M. et al., Multisite Phosphorylation by Cdk2 and GSK3 Controls Cyclin E Degradation. Mol Cell, 12: 381-392, 2003. 4. Stamatakos M. et al., Cell Cyclins: triggering elements of cancer or not? World J Surg Oncol. 8:111, 2010