Cyclin E2, NT (G1/S-specific Cyclin-E2, CCNE2)

Regulated progression through the cell cycle requires sequential expression of a family of proteins called cyclins. Upon their induction, cyclins form complexes with specific cyclin-dependent kinases (CDKs), creating active holoenzymes that phosphorylate target proteins that are required for cell-cycle progression. In higher eukaryotes, CDKl associated with mitotic cyclins (cyclin A, B1, and B2) controls the G2/M transition, while multiple CDK-cyclin complexes control the GUS transition. Putative G1 cyclins (cyclin C, D, and E) were isolated when human and Drosophila cDNA libraries were used to complement yeast G1 mutants. Among the human G1 cyclins, only cyclin E was shown to fluctuate periodically through the cell cycle. The amounts of cyclin E mRNA, protein, and CDK2-associated protein kinase activity were maximal in late G1 in HeLa cells. Functional analysis suggested that cyclin E is rate-limiting for G1 progression, since overexpression of cyclin E accelerated the GUS transition in mammalian fibroblasts, while microinjection of anti-cyclin E antibodies inhibited this transition. In addition, cyclin E was shown to be required for S phase entry and to specifically induce transcription of S phase genes (DNA polymerase a, PCNA, and two ribonucleotide reductase subunits) in Drosophila. It was also reported that multiple levels of regulation of the cyclin E mRNA, protein, and associated kinase activity are present during the first 24h of zebrafish embryonic development.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\nImmunohistochemistry: 1:50-1:200\nELISA: 1:5000-1:20,000\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: C8455-30Q\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C8455-30H

Size

250ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

ZF/Fish

Form

Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Pines, J. (1993) TIBS 18:195-197. 2. van den Heuvel, S., and Harlow, E. (1993) Science 62:2050-2054. 3. Lahue, E.E., et al. (1991) Genes Dev. 5:2166-2175. 4. Lew, D., Dulic, V., and Reed, S.I. (1991) Cell 66: 1197-1206. 5. Dulic, V., Lees, E., and Reed, S.I. (1992) Science 257:1958-1961. 6. Resnitzky, D., et al. (1994) Mol. Cell. Biol. 14:1669-1679. 7. Ohtsubo, M., et al. (1995) Mol. Cell. Biol. 15:2612-2624. 8. Duronio, R.J., and OFarrell, P.H. (1994) Development 120: 1503-1515. 9. Knoblich, J.A., et al. (1994) Cell 77:107-120 10. Yarden, A. and Geiger, B. (1996) Dev. Dyn. 206:1-11

Additional Info

Recognizes zebrafish cyclin E2.