Cyclin T1 pAb

Cyclin T1 was identified as a partner for Cdk9, an RNA polymerase II (RNAPII) transcription elongation factor. Cyclin T1 interacts with the transactivation domain of the HIV-1 Tat protein. The interaction of Tat with cyclin T1 enhances the affinity of Tat for the viral TAR RNA stem-loop structure, suggesting that Tat can recruit cyclin T1/cdk9 to RNAPII through cooperative binding to TAR. The human positive transcription elongation factor b (P-TEFb) consists of a cyclin dependent kinase, cdk9, paired with a cyclin T. Cdk9 may be paired with either cyclin T1 or cyclin T2, in a mutually exclusive manner. Two forms of cyclin T2, T2a and T2b, are due to alternative splicing. The binding of Tat to TAR was shown to be facilitated by human cyclin T1, but not by cyclins T2a or T2b. Cyclin T2 binds to Cdk9 but not to Tat, and cyclin T2 can inhibit cyclin T1-mediated Tat activity.

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SPECIFICATIONS

Catalog Number

BS6875

Size

100ug/100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Recombinant full length Human Cyclin T1.

Species

CCNT1 pAb detects endogenous levels of CCNT1 protein.

Dilutions

WB: 1:500~1:2000\nIHC: 1:50~1:200

Swiss Prot

O60563

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul