Cyclophilin 40

Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents: cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. CyP 40, a 40kD protein, shares significant homology with smaller CyPA (CyP 18) and FKBP59. CyP 40 exhibits the characteristic CsA binding and isomerase activity of CyP 18, though these activities appear to be less with CyP 40 than with Cyp 18. Like FKBP59, CyP 40 has been found in progesterone receptor complexes. CyP 40 is expressed at similar levels in many tissues.\n\nApplications: \nSuitable for use in GS, Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C8856-02A

Size

100ul

Applications

ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.

P Type

Pab

Purity

Whole antisera

Isotype

IgG

References

This product has been used in:Zou J et al. Repression of heat shock transcription factor HSF1 activation by HSP90 (HSP90 complex) that forms a stress-sensitive complex with HSF1. Cell 94:471-80 (1998). <PUBMED:9727490>Hoffmann K & Handschumacher RE Cyclophilin-40: evidence for a dimeric complex with hsp90. Biochem J 307 (Pt 1):5-8 (1995). <PUBMED:7717993>Owens-Grillo JK et al. The cyclosporin A-binding immunophilin CyP-40 and the FK506-binding immunophilin hsp56 bind to a common site on hsp90 and exist in independent cytosolic heterocomplexes with the untransformed glucocorticoid receptor. J Biol Chem 270:20479-84 (1995). <PUBMED:7657624>Milad M et al. Interaction of the progesterone receptor with binding proteins for FK506 and cyclosporin A. Mol Endocrinol 9:838-47 (1995). <PUBMED:7476967> Hutchison KA et al. The 23-kDa acidic protein in reticulocyte lysate is the weakly bound component of the hsp foldosome that is required for assembly of the glucocorticoid receptor into a functional heterocomplex with hsp90. J Biol Chem 270:18841-7 (1995). <PUBMED:7642537>Kieffer LJ et al. Cyclophilin-40, a protein with homology to the P59 component of the steroid receptor complex. Cloning of the cDNA and further characterization. J Biol Chem 268:12303-10 (1993). <PUBMED:8509368>Kieffer LJ et al. Isolation and characterization of a 40-kDa cyclophilin-related protein. J Biol Chem 267:5503-7 (1992). <PUBMED:1544925>

Additional Info

Detects cyclophilin 40 (CyP 40) from human and rat tissues and cells. This antibody does not crossreact with CyPA. Species Crossreactivity: Crossreacts with Human and Rat. Not yet tested in other species.