Cyclophilin A (Cyclosporin A Binding Protein, Peptidyl Prolyl Cis Trans Isomerase A, PPIASE)

Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents-cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). \nBoth CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. \nWithin the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells. Cyclophilin A is a cyclosporin A binding protein that catalyzes the isomerization of proline imidic peptide bonds and is also known as peptidyl-prolyl cis-trans isomerase A (PPIASE). As a cytoplasmic enzyme, it accelerates the folding of proteins.\n\nApplications:\nSuitable for use in Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 1:150\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C8850-05

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, before the addition of glycerol to 40%.

P Type

Pab

Purity

Purified by ammonium sulfate precipitation.

Isotype

IgG

References

1. Haendler, B., et al., EMBO J. 6: 947-950 (1987). 2. Pfuegel, G., et al., Nature 361: 91-94 (1993). 3. J. Biol. Chem. 272(10): 6677-6684 (1997). 4. J. Biol. Chem. 269(29): 18,877-18,880 (1994). 5. J. Virol. 79(1): 176-183 (2005). 6. Toxicological Sciences 79: 266-277 (2004). 7. J. Viol. 77: 9052-9068 (2003). 8. Ren

Additional Info

Recognizes recombinant human Cyclophilin A at ~18kD, as well as CyPa protein from CHO cells. Does not detect endogenous levels. Species Crossreactivity: Hamster.